Target
Human GJC1 / CX45 / Connexin 45
Synonyms
GJC1 | Connexin-45 | Connexin 45 | Gap junction alpha-7 protein | Gap junction gamma-1 protein | CX45 | Gap junction protein alpha-6 | GJA7
Reactivity
Human
(tested or 100% immunogen sequence identity)
Immunogen
A synthetic peptide (QAYSHQN NPHGPRE) as part of human Connexin-45 protein (aa : 354-367) conjugated to diphtheria toxoid.
Specificity
Western blotting of cell membranes of COS cells transiently transfected with cDNA encoding Connexin-45 shows a singular band of molecular weight 47kD which is abolished by preincubation with the immunizing peptide. No such band is detected in western blots of membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-37, Connexin-40 or Connexin-45. Species cross-reactivity: This antiserum reacts to human Connexin-45.