Target
Mouse GJA8 / CX50 / Connexin 50
Synonyms
GJA8 | Connexin 49 | Connexin-50 | CAE | CX50 | Gap junction alpha-8 protein | Gap junction protein alpha 8 | Lens fiber protein MP70 | MP70 | CAE1 | Cell surface glycoprotein | Connexin 50 | CZP1
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
A 13 AA peptide sequence within the 2nd cytoplasmic domain (between 2nd and 3rd transmembrane domain) of Mouse Cx50 (2) was used to generate anti-Mouse Cx50. Peptide was coupled to KLH (carrier protein).
Epitope
2nd Cytoplasmic Domain
Specificity
Recognizes mouse Connexin 50 (CX50, Gap Junction Alpha 8 Protein, CXA-8, Lens Fiber Protein MP70). Species sequence homology: rat 100%. No reactivity observed with other connexins.
Applications
- Western blot (1 - 10 µg/ml)
- ELISA (1:10)
Usage
Suitable for use in Western Blot and ELISA. Western Blot: 1-10 ug/ml using Chemiluminescence technique. The antibodies detected proteins of ~63-65kD in rat lens. The same set of bands were detected by anti-MP70. ELISA: 1:10K-100K using 50-100 ng of the control peptide per well.
Presentation
PBS, pH 7.4, 0.1% BSA, 0.02% sodium merthiolate, 40% glycerol.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GJA8 / CX50 / Connexin 50