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Catalog Number Size Price
LS-C19101-100 100 µg (1.35 mg/ml) $567 
GGA1 Antibody - Anti-GGA1 Antibody - Western Blot. Western blot of Affinity Purified anti-GGA1 antibody shows detection of bands at ~100 kD corresponding to YFP-GGA1 fusion present in a lysate of HEK293 cells over- expressing the recombinant protein (arrowhead). Approximately 35 ug of lysate was separated on a 4-20% gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1350. Reaction occurred overnight at 4? followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Polyclonal Rabbit anti‑Human GGA1 Antibody (WB) LS‑C19101

Polyclonal Rabbit anti‑Human GGA1 Antibody (WB) LS‑C19101

Note: This antibody replaces LS-C66860
Antibody:
GGA1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C19101-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
GGA1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
GGA1 antibody LS-C19101 is an unconjugated rabbit polyclonal antibody to human GGA1. Validated for ELISA, IP and WB.
Target
Human GGA1
Synonyms
GGA1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 330-360 of Human GGA-1.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. Cross reactivity may occur with GGA1 from bovine (87% homology), mouse (82% homology) and rat (76% homology) sources. The immunizing sequence is present only in isoform 1 of this protein, therefore cross reactivity with splice variants is not expected. Cross reactivity with GGA1 homologues from other sources has not been determined.
Applications
  • Western blot (1:500 - 1:3000)
  • Immunoprecipitation (1:100)
  • ELISA (1:10000 - 1:50000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at 70 kD in size corresponding to GGA1 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GGA1
Q9UJY5 NM_013365 NP_037497.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/27/2024