Pig GFAP

Mouse

Pig, Human, Rat, Chicken (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.

Protein G purified

Unmodified

Porcine spinal cord. Cellular Localization: Cytoplasmic.

Recognizes porcine glial fibrillary acidic protein (GFAP) at 51-52kD. Species cross-reactivity: human, rat and chicken.

• IHC
• IHC - Paraffin (1 - 2 µg/ml)
• Immunofluorescence
• Western blot (1 - 2 µg/ml)
• Immunoprecipitation (2 µg/tst)

Immunohistochemistry: LS-B2801 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2801 was determined to be 1-2 ug/ml. Immunoprecipitation (Native and denatured): 2 ug/mg protein lysate (Use Protein G). Western Blot: 1-2 ug/ml for 2 hrs at RT. Positive control: Astrocytoma, IMR5 and brain cells.

PBS, pH 7.4, 0.1% Sodium Azide, 40% Glycerol, 0.2% BSA

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14136 NCBI: NM_002055 NP_002046.1