Mouse GDF5 / GDF-5

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein G purified

Unmodified

E. coli-derived monomeric rmGDF-5.

Recognizes mouse GDF-5 in direct ELISA. Does not cross-react with rmGDF-1, rmGDF-3, or rmGDF-8.

• ELISA (0.5 - 1 µg/ml)
• Functional Assay
• Neutralization

Suitable for use in direct ELISA and Neutralization. Neutralization (ND50): ~2-10 ug/ml in the presence of 3 ug/ml of rmGDF-5, using the ATDC5 cell line. Note: The exact concentration of antibody required to neutralize rmGDF-5 activity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Direct ELISA: 0.5-1.0 ug/ml. The detection limit is ~10 ng/well.

Lyophilized from PBS, 5% Trehalose

Reconstitute in sterile PBS.

Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P43026 NCBI: NM_000557 NP_000548.2