Human GDF2 / BMP9

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

360107

Unconjugated

Affinity purified

Unmodified

Recombinant protein corresponding to aa320-429 from human BMP-9, expressed in E.coli (UniProt# Q9UK05).

Recognizes human Bone Morphogenetic Protein 9. Does not cross react with recombinant human BMP-1, -1.1, -2, -3, -3b, -4, -5, -6, -7, -8 or -10. In direct ELISAs, ~80% cross-reactivity with recombinant mouse BMP-9 and 10% cross-reactivity with recombinant mouse BMP-10 is observed.

• Western blot (1 - 2 µg/ml)
• ELISA (0.5 - 1 µg/ml)
• Functional Assay
• Neutralization

Suitable for use in Western Blot, ELISA and Neutralization. Western Blot: 1-2 ug/ml; The detection limit is ~5 ng/lane under non-reducing conditions. Direct ELISA: 0.5-1.0 ug/ml; The detection limit is ~5 ng/well. Neutralization of human BMP-9 bioactivity: The ND50 is defined as the concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit maximum response. The ND50 of this lot is ~0.01-0.04 ug/ml in the presence of 2 ng/ml rhBMP-9 using ATDC5 chondrogenic cells.

Lyophilized from Sterile Solution PBS, 5% Trehalose

Reconstitute in sterile PBS.

Store at -20°C to -70°C for up to 1 year.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q9UK05 NCBI: NM_016204 NP_057288.1