Target
Human GC / Vitamin D-Binding Protein
Synonyms
GC | Gc-globulin | HDBP | Group-specific component | GRD3 | VDB | VDBG | VDBP | Vitamin D-binding protein
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Purified hVitD BP from human plasma.
Specificity
Detects human VitD BP in direct ELISAs and Western Blots.
Applications
- Western blot (1 - 2 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 1-2 ug/ml. Using a colorimetric detection system limit ~2 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 ug/ml. The detection limit ~10 ng/well. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, VitD BP from human plasma.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute in sterile PBS.
Storage
Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GC / Vitamin D-Binding Protein