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Antibodies > GATA4 > LS-C353987
 
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Catalog Number Size Price
LS-C353987-100 100 µl (1 mg/ml) $299 
GATA4 Antibody - Immunohistochemical analysis of GATA4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
GATA4 Antibody - Immunofluorescent analysis of GATA4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
GATA4 Antibody - Western blot analysis of GATA4 expression in HepG2 (A); HeLa (B) whole cell lysates.
GATA4 Antibody - Immunohistochemical analysis of GATA4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
GATA4 Antibody - Immunofluorescent analysis of GATA4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
GATA4 Antibody - Western blot analysis of GATA4 expression in HepG2 (A); HeLa (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human GATA4 Antibody (Internal, IHC, IF, WB) LS‑C353987

Polyclonal Rabbit anti‑Human GATA4 Antibody (Internal, IHC, IF, WB) LS‑C353987

Submit Review LSBio Guarantee
Antibody:
GATA4 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Dog, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C353987-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GATA4 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Dog, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
GATA4 antibody LS-C353987 is an unconjugated rabbit polyclonal antibody to GATA4 (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human GATA4
Synonyms
GATA4 | ASD2 | GATA-binding factor 4 | GATA-binding protein 4 | GATA binding protein 4 | Transcription factor GATA-4 | VSD1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human GATA4.
Epitope
Internal
Specificity
Recognizes endogenous levels of GATA4 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GATA4
P43694 NM_002052 NP_002043.2

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Featured Products

GATA4 Antibody - IHC of paraffin-embedded Human endometrium tissue using anti-GATA4 mouse monoclonal antibody.
GATA4 Antibody (clone OTI9F9) LS‑C114374
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
GATA4 Antibody - Anti-GATA4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
IHC‑plus™ GATA4 Antibody (aa330‑380) LS‑B7989
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
GATA4 Antibody - Immunohistochemical analysis of GATA4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
GATA4 Antibody (Internal) LS‑C352237
Species: Human, Mouse, Rat, Dog, Pig, Chicken
Applications: IHC, IHC - Paraffin, Western blot
GATA4 Antibody - Western blot of recombinant GATA4.
GATA4 Antibody (aa201‑442, PE) LS‑C721267
Species: Human
Applications: Western blot
GATA4 Antibody - Detection of Human GATA4 by Immunohistochemistry. Sample: FFPE section of human stomach linitis plastica. Antibody: Affinity purified rabbit anti-GATA4 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
GATA4 Antibody (aa392‑442) LS‑C290176
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
GATA4 ELISA Kit
Mouse/Human Phospho-GATA4 (Ser262) (DNA-Binding Phosphorylation ELISA) ELISA Kit - LS-F877
Reactivity: Human, Mouse
Range:
See all GATA4 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/27/2024
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Tel: (800) 227-6666 / (650) 697-3600

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For technical assistance:
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