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Antibodies > GATA1 > LS-C352235
 
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Catalog Number Size Price
LS-C352235-100 100 µl (1 mg/ml) $299 
GATA1 Antibody - Immunohistochemical analysis of GATA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
GATA1 Antibody - Immunofluorescent analysis of GATA1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
GATA1 Antibody - Western blot analysis of GATA1 expression in HeLa (A); K562 (B); NIH3T3 (C) whole cell lysates.
GATA1 Antibody - Immunohistochemical analysis of GATA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
GATA1 Antibody - Immunofluorescent analysis of GATA1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
GATA1 Antibody - Western blot analysis of GATA1 expression in HeLa (A); K562 (B); NIH3T3 (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human GATA1 Antibody (Internal, IHC, IF, WB) LS‑C352235

Polyclonal Rabbit anti‑Human GATA1 Antibody (Internal, IHC, IF, WB) LS‑C352235

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Antibody:
GATA1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352235-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
GATA1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
GATA1 antibody LS-C352235 is an unconjugated rabbit polyclonal antibody to GATA1 (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB.
Target
Human GATA1
Synonyms
GATA1 | ERYF1 | Globin transcription factor 1 | GATA-binding factor 1 | Erythroid transcription factor | GATA-1 | GF-1 | NFE1 | NF-E1 DNA-binding protein | Transcription factor GATA1 | XLTDA | XLTT | GF1 | XLANP
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human GATA1.
Epitope
Internal
Specificity
Recognizes endogenous levels of GATA1 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GATA1
P15976 NM_002049 NP_002040.1

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Featured Products

GATA1 Antibody - Western blot analysis of GATA1 (pS310) expression in HeLa (A); HEK293T (B) whole cell lysates.
GATA1 Antibody (C‑Terminus) LS‑C356051
Species: Human, Monkey, Mouse, Rat, Pig, Sheep
Applications: ICC, Immunofluorescence, Western blot
GATA1 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using GATA1 Antibody. The picture on the right is blocked with the synthesized peptide.
GATA1 Antibody (aa109‑158) LS‑C118126
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, Peptide Enzyme-Linked Immunosorbent Assay
GATA1 Protein - Recombinant GATA Binding Protein 1 By SDS-PAGE
Mouse GATA1 Protein (Recombinant His-T7) (aa20-265) - LS-G12022
Source: E. coli
Tag: His-T7
See all GATA1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
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For technical assistance:
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