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Catalog Number Size Price
LS-C482441-50 50 µl (1 mg/ml) $286 
LS-C482441-100 100 µl (1 mg/ml) $321 
LS-C482441-200 200 µl (1 mg/ml) $386 
GARS / Glycyl tRNA Synthetase Antibody - Immunohistochemical analysis of GlyRS staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
GARS / Glycyl tRNA Synthetase Antibody - Immunofluorescent analysis of GlyRS staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
GARS / Glycyl tRNA Synthetase Antibody - Western blot analysis of GlyRS expression in Jurkat (A); Raji (B); HT29 (C); mouse brain (D) whole cell lysates.
GARS / Glycyl tRNA Synthetase Antibody - Immunohistochemical analysis of GlyRS staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
GARS / Glycyl tRNA Synthetase Antibody - Immunofluorescent analysis of GlyRS staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
GARS / Glycyl tRNA Synthetase Antibody - Western blot analysis of GlyRS expression in Jurkat (A); Raji (B); HT29 (C); mouse brain (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human GARS / Glycyl tRNA Synthetase Antibody (IHC, IF, WB) LS‑C482441

Polyclonal Rabbit anti‑Human GARS / Glycyl tRNA Synthetase Antibody (IHC, IF, WB) LS‑C482441

Antibody:
GARS / Glycyl tRNA Synthetase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$286
LS-C482441-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
GARS / Glycyl tRNA Synthetase Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Glycyl tRNA Synthetase antibody LS-C482441 is an unconjugated rabbit polyclonal antibody to Glycyl tRNA Synthetase (GARS) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human GARS / Glycyl tRNA Synthetase
Synonyms
GARS | AP-4-A synthetase | CMT2D | DSMAV | Glycine--tRNA ligase | Glycine tRNA ligase | Glycyl-tRNA synthetase | GlyRS | HMN5
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Recombinant full length human GlyRS.
Specificity
Recognizes endogenous levels of GlyRS protein.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GARS / Glycyl tRNA Synthetase
P41250 NM_002047 NP_002038.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 12/30/2024