Target
Mouse FZD7 / Frizzled 7
Synonyms
FZD7 | Frizzled family receptor 7 | Frizzled homolog 7 | Frizzled 7 | Frizzled-7 | FzE3 | Fz-7 | HFz7
Reactivity
Mouse, Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
NS0-derived rmFrizzled-7 extracellular CRD domain.
Specificity
Recognizes mouse Frizzled-7. Shows no cross-reactivity with rmFrizzled-1, -3, -4 or -8. Species cross-reactivity: human.
Applications
- ICC (10 µg/ml)
- Flow Cytometry
- ELISA
Usage
Suitable for use in Flow Cytometry, Direct ELISA and Immunocytochemistry. Flow Cytometry: Dilute to 5-10 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunocytochemistry: 10 ug/ml with the appropriate secondary reagents in 4% paraformaldehyde-fixed cells.
Presentation
Lyophilized from PBS, pH 7.2, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee