Mouse FZD6 / Frizzled 6

Goat

Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

NS0-derived rmFrizzled-6 extracellular Cysteine-Rich Domain (CRD).

Recognizes mouse Frizzled-6 extracellular Cysteine-Rich Domain (CRD). Less than 1% cross-reactivity with rmFrizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, rmFrizzled-7, rmFrizzled-8 and rmFrizzled-9.

• IHC
• IHC - 4% paraformaldehyde (5 - 10 µg/ml)
• Western blot (0.1 - 0.2 µg/ml)
• Flow Cytometry
• ELISA (0.5 - 1 µg/ml)

Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Between each step, cells were washed with 0.1% BSA in PBS. Flow Cytometry: 0.1 mg/ml. Use 10 ul to label 1-2.5x10^5 cells in a total volume <200ul. The binding of unlabeled primary antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of IgG (FITC) xGt.

Lyophilized from PBS, pH 7.4, 5% Trehalose

Reconstitute with 500ul sterile PBS

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O60353 NCBI: NM_003506 NP_003497.2