Target
Human FZD3 / Frizzled 3
Synonyms
FZD3 | Frizzled family receptor 3 | Frizzled 3 | HFz3 | Frizzled homolog 3 | Fz-3 | Wnt receptor frizzled-3 | Frizzled-3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DDQRSKAGSIHSK, from the internal region of the protein sequence according to NP_059108.1.
Specificity
Human FZD3 / Frizzled 3.
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:2000)
Usage
Peptide ELISA: antibody detection limit dilution 1:2000. Western Blot: Preliminary experiments in Human Brain (Frontal Cortex), Mouse Brain and Rat Brain lysates gave no specific signal but low background (at antibody concentration up to 1 ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee