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Catalog Number	Size	Price
LS-C355415-1	1 mg	$801

FSH Antibody - Immunofluorescence analysis of Follicle Stimulating Hormone was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Follicle Stimulating Hormone (1G6D12) Mouse Monoclonal Antibody at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

FSH Antibody - Western blot analysis was performed on whole cell extracts (30 µg lysate) of SK-OV-3 (Lane 1). The blot was probed with Anti-Follicle Stimulating Hormone Mouse monoclonal and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate. A ~21 kDa band corresponding to Follicle Stimulating Hormone was observed in the cell line tested. Known quantity of protein samples were electrophoresed using 10 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody.

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Monoclonal Mouse anti‑Human FSH Antibody (clone 1G6D12, IF, WB) LS‑C355415

Name: Anti-FSH Antibody | Mouse anti-Human Monoclonal ELISA,IF,IP,RIA,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C355415-1
Price: 801.0000 USD
Availability: InStock

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Antibody:

FSH Mouse anti-Human Monoclonal (1G6D12) Antibody

Application:

IF, WB, IP, ELISA, RIA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$801

LS-C355415-1

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Overview

Antibody:

FSH Mouse anti-Human Monoclonal (1G6D12) Antibody

Application:

IF, WB, IP, ELISA, RIA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

FSH antibody LS-C355415 is an unconjugated mouse monoclonal antibody to human FSH. Validated for ELISA, IF, IP, RIA and WB.

Target

Human FSH

Synonyms

FSH

See All FSH Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG2a Monoclonal

Clone

1G6D12

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Immunogen

Human Follicle Stimulating Hormone

Specificity

The antibody targets Follicle Stimulating Hormone in ELISA, IP, and RIA applications and shows reactivity with Human samples. It detects Follicle Stimulating Hormone which has a predicted molecular weight of approximately 13 kD.

Applications