Target
Mouse FOXP3
Synonyms
FOXP3 | AIID | DIETER | Forkhead box protein P3 | JM2 | Forkhead box P3 | FOXP3delta7 | XPID | PIDX | SCURFIN
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
A fusion protein, GST-mouse Foxp3-Flag. Mouse Foxp3 Gly43 to His100 peptide and Flag Tag were fused at C-Terminus of GST protein.
Specificity
Species cross-reactivity: This antibody has shown reactivity against mouse protein (CD4+CD25+ T cells). There appears to be no reactivity against human Jurkat and mouse CD4+CD25- T cell lysates. Other species and samples have not been tested.
Applications
- ICC
- Western blot
- Flow Cytometry
Usage
Suitable for use in FACS/Flow Cytometry, Western Blot and Immunocytochemistry. Recommended Dilutions Western Blot: Band is seen at ~48kD in FOXP3 transfected and CD25/CD4 positive regulatory T cell lysates. Positive Control: Mouse CD4+CD25+ T cells
Presentation
Tris-Glycine, NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee