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Catalog Number	Size	Price
LS-C368596-30	30 µl (1 mg/ml)	$263
LS-C368596-100	100 µl (1 mg/ml)	$315
LS-C368596-200	200 µl (1 mg/ml)	$379

FOXO3 / FOXO3A Antibody - Immunohistochemical analysis of FOXO3 (pS253) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

FOXO3 / FOXO3A Antibody - Immunofluorescent analysis of FOXO3 (pS253) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

FOXO3 / FOXO3A Antibody - Western blot analysis of FOXO3 (pS253) expression in HeLa (A); Raw264.7 (B); mouse liver (C); rat smooth muscle (D) whole cell lysates.

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Polyclonal Rabbit anti‑Human FOXO3 / FOXO3A Antibody (phospho‑Ser253, IHC, IF, WB) LS‑C368596

Name: Anti-FOXO3 / FOXO3A Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C368596-30
Price: 263.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

FOXO3 / FOXO3A Rabbit anti-Human Polyclonal (pSer253) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$263

LS-C368596-30

$315

LS-C368596-100

$379

LS-C368596-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

FOXO3 / FOXO3A Rabbit anti-Human Polyclonal (pSer253) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

FOXO3A antibody LS-C368596 is an unconjugated rabbit polyclonal antibody to FOXO3A (FOXO3) (pSer253) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC, IP and WB.

Target

Human FOXO3 / FOXO3A

Synonyms

See All FOXO3 / FOXO3A Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the central region of human FOXO3.

Epitope

pSer253

Specificity

Recognizes endogenous levels of FOXO3 (pS253) protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:10 - 1:100)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About FOXO3 / FOXO3A

Uniprot: O43524 NCBI: NM_001455 NP_001446.1