Target
Human FOLR1 / Folate Receptor Alpha
Synonyms
FOLR1 | Adult folate-binding protein | FBP | Folate receptor alpha | KB cells FBP | Folate binding protein | Folate receptor 1 | Folate receptor 1 (adult) | Folate receptor, adult | FOLR | FR-alpha
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
Purified, CHO-derived, recombinant human FOLR1 (rhFOLR1; aa 25-233; Accession# P15328).
Specificity
Recognizes human FOLR1. In Direct ELISA, shows no cross-reactivity with recombinant human FOLR2, 3 or 4. Species sequence Homology: mouse, 83%.
Applications
- ICC
- Western blot
- Flow Cytometry (25 µg/ml)
- ELISA (0.5 - 1 µg/ml)
Usage
ELISA (Capture): 2-8ug/mlFlow Cytometry: 2.5ug labels 10e6 cells. Tested in MCF-7 cells.Western Blot: 2ug/ml detects human FOLR1 in human cortex tissue and HeLa cells under non-reducing conditions only. Immunocytochemistry: 8-25ug/ml. Tested on immersion fixed MCF-7 cells.Optimal dilutions to be determined by the researcher.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
Reconstitute in 200 ul sterile PBS
Storage
Store lyophilized at -20C. After reconstitution, store at -20C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FOLR1 / Folate Receptor Alpha