Conjugations
Tetramethyl Rhodamine Iso-Thiocyanate
Purification
Affinity chromatography
Immunogen
Fluorescein conjugated to Goat IgG.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum and fluorescein conjugated IgG.
Applications
- Immunofluorescence (1:500 - 1:2500)
- Western blot (1:10000)
- Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee