Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Immunogen
Fluorescein conjugated Bovine Serum Albumin (BSA).
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Fluorescein conjugated goat IgG and Fluorescein conjugated KLH.
Applications
- IHC
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
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Usage
This product has been assayed against 1.0 ug of Fluorescein conjugated Rabbit IgG in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Donkey) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100000 to 1:500000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Fluorescein conjugated Rabbit IgG in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Donkey) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100000 to 1:500000 of the reconstitution concentration is suggested for this product.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee