Mouse Fibrinogen

Goat

Mouse (tested or 100% immunogen sequence identity)

Polyclonal

Biotin. Also available Unconjugated or conjugated with FITC, HRP.

Purified IgG

Unmodified

Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.

Mouse fibrinogen.

• IHC
• IHC - Paraffin
• ICC
• ELISA
• Dot Blot
• Immunodiffusion
• Applications tested for the base form of this product only

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As reagent for the direct detection of fibrinogen in mouse cells, tissues and body fluids in immunocytochemical and immunohistochemical assays. as detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA). As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays are between 1:1000 and 1:8000.

Lyophilized from PBS, pH 7.2, No preservatives added

1 ml Distilled water

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.