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Catalog Number	Size	Price
LS-C97524-200	200 µl	$393

FGFR4 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

FGFR4 Antibody - Fluorescent confocal image of HeLa cells stained with FGFR4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated FGFR4 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the FGFR4 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000160867).

FGFR4 Antibody - FGFR4 Antibody (E39) western blot of 293 cell line lysates (35 ug/lane). The FGFR4 antibody detected the FGFR4 protein (arrow).

FGFR4 Antibody - Western blot of lysates from SH-SY5Y 293, Raji cell line (from left to right), using FGFR4 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

FGFR4 Antibody - Flow cytometric of WiDr cells using FGFR4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Polyclonal Rabbit anti‑Human FGFR4 Antibody (aa24‑55, IHC, IF, WB) LS‑C97524

Name: Anti-FGFR4 Antibody | Rabbit anti-Human Polyclonal Flo,IF,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C97524-200
Price: 393.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

FGFR4 Rabbit anti-Human Polyclonal (aa24-55) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$393

LS-C97524-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

FGFR4 Rabbit anti-Human Polyclonal (aa24-55) Antibody

Application:

IHC, IHC-P, IF, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

FGFR4 antibody LS-C97524 is an unconjugated rabbit polyclonal antibody to human FGFR4 (aa24-55). Validated for Flow, IF, IHC and WB.

Target

Human FGFR4

Synonyms

See All FGFR4 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Ammonium sulfate precipitation

Modifications

Unmodified

Epitope

aa24-55

Specificity

This FGFR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-55 amino acids from the N-terminal region of human FGFR4.

Applications

IHC
IHC - Paraffin (1:50 - 1:100)
Immunofluorescence (1:200)
Western blot (1:1000)
Flow Cytometry (1:10 - 1:50)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, 0.09% Sodium Azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About FGFR4

Uniprot: P22455 NCBI: NM_002011 NP_002002.3