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Catalog Number Size Price
LS-C390083-20 20 µg (0.2 mg/ml) $371 
LS-C390083-100 100 µg (0.2 mg/ml) $574 
FCGR2 / CD32 Antibody - SDS-PAGE Analysis of Purified, BSA-Free CD32 Antibody (clone 8.7). Confirmation of Integrity and Purity of the Antibody.

Monoclonal Mouse anti‑Human FCGR2 / CD32 Antibody (clone 8.7, IF) LS‑C390083

Monoclonal Mouse anti‑Human FCGR2 / CD32 Antibody (clone 8.7, IF) LS‑C390083

Antibody:
FCGR2 / CD32 Mouse anti-Human Monoclonal (8.7) Antibody
Application:
IF, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$371
LS-C390083-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
FCGR2 / CD32 Mouse anti-Human Monoclonal (8.7) Antibody
Application:
IF, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD32 antibody LS-C390083 is an unconjugated mouse monoclonal antibody to human CD32 (FCGR2). Validated for Flow and IF.
Target
Human FCGR2 / CD32
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
8.7
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Azide-free, BSA-free.
Immunogen
K562 and FcgRII+L cells were used as the immunogen for the CD32 antibody.
Specificity
Human FCGR2 / CD32.
Applications
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Usage
This mAb reacts with a CD32 (FcgRII) epitope distinct from that defined by mAb 8.26 and the epitope overlaps with that of mAb 7.30 (cluster 4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcgRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes. Optimal dilution of the CD32 antibody should be determined by the researcher.
Presentation
1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024