Target
Human FCGR1A / CD64
Synonyms
FCGR1A | CD64A | CD64 | CD64 antigen | FCG1 | FcgammaRIa | IGFR1 | IgG Fc receptor I | Fc-gamma receptor I A1 | Fc-gamma RIA | FCGR1 | Fc gamma receptor | Fc-gamma RI | FCRI
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Fab.
Also available
Unmodified or
Fab'2,
Fab,
Azide-free.
Immunogen
Human rheumatoid synovial fluid cells/ monocytes.
Specificity
CD64 molecule of 72 kd from gene Fc gamma RIA.
Applications
- Western blot
- Flow Cytometry
Usage
Five x 105 cultured U-937 cells were washed and incubated 45 minutes on ice with 80 ^l of antiCD64 Fab at a concentration of 10 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.4 log10 fluorescent units when compared to a Mouse IgG1 Fab negative control at a similar concentration. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
Sterile Solution 50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, No preservatives added
Storage
Store at 4°C. Do not freeze. Open under aseptic conditions.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee