Yeast FBL / FIB / Fibrillarin

Mouse

Yeast, Human, Rat (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

38F3

Unconjugated

Tissue culture supernatant

Unmodified

Mice were injected with yeast nuclear preparations and hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates.

This clone stains a single ~34kDa band on western blotting and shows a clear and strong punctate staining of yeast nuclei. Subsequently it was found that this antibody recognizes fibrillarin in a wide variety of species including human, rat, Drosophila, S. pombe, C. elegans, and plants. It can therefore be used to identify nucleoli immunocytochemically.

• ICC (1:1000 - 1:5000)
• Immunofluorescence (1:1000 - 1:5000)
• Western blot (1:2000 - 1:10000)

For western blots of yeast protein samples, use FBL / FIB / Fibrillarin antibody diluted 1:2000 (cell lysates) to 1:10000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods, try the antibody diluted 1:1000-1:5000. To detect mammalian fibrillarin on western blots by ECL, try the antibody at 1:500 dilution. For immunofluorescence on yeast cells, use the antibody diluted 1:1000-1:5000. For IF of mammalian cells, try the antibody at 1:500.

Tissue Culture Supernatant, 10 mM Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P22087 NCBI: NM_001436 NP_001427.2