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Catalog Number Size Price
LS-C662101-10 10 µg $318 
LS-C662101-100 100 µg $470 
FABP4 / AP2 Antibody - Western blot analysis of FABP4 using anti-FABP4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysate,Lane 2: mouse cardiac muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FABP4 at approximately 15KD. The expected band size for FABP4 is at 15KD.

Monoclonal Rabbit anti‑Rat FABP4 / AP2 Antibody (WB) LS‑C662101

Monoclonal Rabbit anti‑Rat FABP4 / AP2 Antibody (WB) LS‑C662101

Antibody:
FABP4 / AP2 Rabbit anti-Rat Monoclonal Antibody
Application:
WB, ELISA
Reactivity:
Rat, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662101-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
FABP4 / AP2 Rabbit anti-Rat Monoclonal Antibody
Application:
WB, ELISA
Reactivity:
Rat, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
AP2 antibody LS-C662101 is an unconjugated rabbit monoclonal antibody to AP2 (FABP4) from rat. It is reactive with mouse and rat. Validated for ELISA and WB.
Target
Rat FABP4 / AP2
Synonyms
FABP4 | A-FABP | AFABP | ALBP | AP2 | Fatty acid-binding protein 4
Host
Rabbit
Reactivity
Rat, Mouse (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived rat FABP4 recombinant protein (Position: C2-A132). Rat FABP4 shares 88.5% and 93.9% amino acid (aa) sequence identity with human and mouse FABP4, respectively.
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FABP4 / AP2
P15090 NM_001442 NP_001433.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/28/2024