Human Estrogen Receptor

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

NR3Ga-1

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free.

Estrogen Receptor alpha protein (aa 2-185).

aa2-185

Human Estrogen Receptor

• IHC
• IHC - Paraffin (1 - 2 µg/ml)
• Western blot (0.5 - 1 µg/ml)

Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. It is found in two forms, alpha and beta. Both forms are widely expressed, however, alpha is the predominant form in endometrium and breast cancer cells, and beta is more highly expressed in brain, kidney, heart, lung and bone. Activated Estrogen receptor dimerizes and forms both homo- and heterodimers. ER contains five domains: A-F. The A and B domain are able to weakly activate ER in the absence of ligand. The C domain binds DNA and the E domain contains the ligand binding site. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This antibody is specific to estrogen receptor alpha and shows minimal cross-reaction with other members of the family. Titration of the antibody may be required for optimal performance. Staining of FFPE tissues requires boiling sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.