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| Catalog Number | Size | Price |
|---|---|---|
| LS-B945-50 | 50 µl (0.5 mg/ml) | $460 |
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Polyclonal Rabbit anti‑Human ESR2 / ER Beta Antibody (aa82‑131, IHC, WB) LS‑B945
Polyclonal Rabbit anti‑Human ESR2 / ER Beta Antibody (aa82‑131, IHC, WB) LS‑B945
Note: This antibody replaces LS-C30073
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
ER Beta antibody LS-B945 is an unconjugated rabbit polyclonal antibody to human ER Beta (ESR2) (aa82-131). Validated for IHC and WB. Cited in 1 publication.
Target
Human ESR2 / ER Beta
Synonyms
ESR2 | ESTRB | ER Beta | ERbeta | ER-BETA | ESR-BETA | ESRB | Estrogen receptor 2 (ER beta) | NR3A2 | Estrogen receptor beta | Estrogen receptor beta 4
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide located between aa82-131 of human ESR2 (Q92731, NP_001428). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Mouse, Rat, Bovine, Bat, Pig (100%); Galago, Marmoset, Sheep, Panda, Dog, Guinea pig (92%); Hamster, Elephant, Cat (85%); Rabbit (84%).
Epitope
aa82-131
Specificity
Human ESR2 / ER-Beta
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.2 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B945 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B945 was determined to be 5 ug/ml.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ESR2 / ER Beta
LSBio Ratings
ESR2 / ER Beta Antibody (aa82-131) for IHC, WB/Western LS-B945 has an LSBio Rating of
Publications (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Dienogest increases the progesterone receptor isoform B/A ratio in patients with ovarian endometriosis. Hayashi A, Tanabe A, Kawabe S, Hayashi M, Yuguchi H, Yamashita Y, Okuda K, Ohmichi M. Journal of ovarian research. 2012 5:31.
Customer Reviews (0)
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Species:
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ICC, Immunofluorescence, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
Date Requested: 12/26/2024
