Target
Human ERN2
Synonyms
ERN2 | ER to nucleus signalling 2 | HIRE2p | Inositol-requiring protein 2 | IRE1-BETA | Inositol-requiring 1 beta | IRE1 beta | IRE2 | IRE1b
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-QKRLQSPSKQAQPLD, from the internal region of the protein sequence according to NP_150296.3.
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Preliminary experiments gave an approx 37kD band in Human Duodenum, Pancreas and Placenta lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kD according to NP_150296.3. The 37kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee