Target
Human ERN1 / IRE1
Synonyms
ERN1 | ER to nucleus signalling 1 | Inositol-requiring enzyme 1 | Inositol-requiring protein 1 | Ire1-alpha | IRE1a | HIRE1p | IRE1 | Inositol-requiring 1 | IRE1P
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen
Synthetic peptide corresponding to Human IRE1 (phospho S724).
Specificity
IRE1 (phospho S724) Antibody detects IRE1 alpha, phosphorylated (serine 724) protein.
Applications
- Western blot (1:1000 - 1:3000)
- Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee