Reactivity
Human, Monkey, Rat, Pig, Drosophila
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
Synthetic phospho-peptide (KLH coupled) corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase.
Specificity
Recognizes endogenous levels of human p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Tyr204. The antibody does not cross-react with the corresponding
Applications
- Immunofluorescence (1:100)
- Western blot (1:1000)
- Immunoprecipitation
- Flow Cytometry (1:100)
Usage
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Flow Cytometry. Western blotting 1:1000. Immunofluorescence (IF-IC) 1:100. Flow Cytometry 1:100.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee