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Antibodies > ERF / PE2 > LS-C352166
 
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Catalog Number Size Price
LS-C352166-100 100 µl (1 mg/ml) $299 
ERF / PE2 Antibody - Immunohistochemical analysis of PE2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ERF / PE2 Antibody - Immunofluorescent analysis of PE2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ERF / PE2 Antibody - Western blot analysis of PE2 expression in A431 (A); HeLa (B); NIH3T3 (C) whole cell lysates.
ERF / PE2 Antibody - Immunohistochemical analysis of PE2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ERF / PE2 Antibody - Immunofluorescent analysis of PE2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ERF / PE2 Antibody - Western blot analysis of PE2 expression in A431 (A); HeLa (B); NIH3T3 (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human ERF / PE2 Antibody (C‑Terminus, IHC, IF, WB) LS‑C352166

Polyclonal Rabbit anti‑Human ERF / PE2 Antibody (C‑Terminus, IHC, IF, WB) LS‑C352166

Submit Review LSBio Guarantee
Antibody:
ERF / PE2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352166-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ERF / PE2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PE2 antibody LS-C352166 is an unconjugated rabbit polyclonal antibody to PE2 (ERF) (C-Terminus) from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB.
Target
Human ERF / PE2
Synonyms
ERF | PE-2 | Ets2 repressor factor | PE2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human PE2.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of PE2 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ERF / PE2
P50548 NM_006494 NP_006485.2

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Featured Products

ERF / PE2 Antibody - Immunohistochemical analysis of PE2 (pT526) staining in human ovarian cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
ERF / PE2 Antibody (C‑Terminus) LS‑C358748
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Product
ERF / PE2 Antibody (470‑550 aa) LS‑C381711
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, ELISA
ERF / PE2 Antibody - Western blot of ERF antibody
ERF / PE2 Antibody (470‑550 aa) LS‑C383402
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, ELISA
ERF / PE2 Antibody - Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using ERF (Phospho-Thr526) Antibody. The picture on the right is blocked with the phospho peptide.
ERF / PE2 Antibody (phospho‑Thr526) LS‑C198928
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
ERF / PE2 ELISA Kit
Mouse/Human ERF / PE2 (DNA-Binding ELISA) ELISA Kit - LS-F751
Reactivity: Human, Mouse
Range:
ERF / PE2 Protein - 12.5% SDS-PAGE of human ERF stained with Coomassie Blue
Human ERF / PE2 Protein (Recombinant GST) (Full Length) - LS-G28383
Source: Wheat
Tag: GST
See all ERF / PE2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
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6737 Mowry Ave
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For technical assistance:
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