Monoclonal Mouse anti‑Human EMA / MUC1 Antibody (clone SPM492, Azide‑free,BSA‑free, IHC, IF) LS‑C390716
Monoclonal Mouse anti‑Human EMA / MUC1 Antibody (clone SPM492, Azide‑free,BSA‑free, IHC, IF) LS‑C390716
Antibody:
EMA / MUC1 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (SPM492) Antibody
Application:
IHC, IHC-P, IF, Flo, ELISA
Format:
Unconjugated, Azide-free, BSA-free
Overview
Antibody:
EMA / MUC1 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (SPM492) Antibody
Application:
IHC, IHC-P, IF, Flo, ELISA
Format:
Unconjugated, Azide-free, BSA-free
Specifications
Description
MUC1 antibody LS-C390716 is an unconjugated mouse monoclonal antibody to MUC1 (EMA) from human. It is reactive with human and mouse. Validated for ELISA, Flow, IF and IHC.
Target
Human EMA / MUC1
Synonyms
MUC1 | CA15-3 | CD227 | Carcinoma-associated mucin | CD227 antigen | DF3 antigen | Episialin | Epithelial membrane antigen | H23 antigen | H23AG | Krebs von den Lungen-6 | MAM6 | MUC1/ZD | MUC-1/SEC | Mucin-1 | Pem | KL-6 | MUC-1/X | Tumor-associated mucin | Peanut-reactive urinary mucin | Polymorphic epithelial mucin | PUM | EMA | MUC-1 | Mucin 1, transmembrane
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free, BSA-free.
Also available
Unmodified.
Immunogen
Breast cancer cell line ZR75-1.
Specificity
Human EMA / MUC1
Applications
- IHC
- IHC - Paraffin (0.5 - 1 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Flow Cytometry (0.25 - 0.5 µg/10E6 cells)
- ELISA (2 - 5 µg/ml)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. This mAb reacts with MUC1/Mucin-1/Epithelial Marker Antigen/EMA. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Optimal dilution of the anti-MUC-1 antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry
Species:
Human, Mouse, Pig
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 10/19/2024