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Catalog Number Size Price
LS-C407795-10 10 µg $318 
LS-C407795-100 100 µg $470 
EIF6 Antibody - IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-EIF6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
EIF6 Antibody - IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-EIF6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
EIF6 Antibody - EIF6 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.
EIF6 Antibody - EIF6 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
EIF6 Antibody - EIF6 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.
EIF6 Antibody - EIF6 antibody Western blot. All lanes: Anti EIF6 at 0.5 ug/ml. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: Mouse Liver Tissue Lysate at 50 ug. Lane 4: Human Placenta Tissue Lysate at 50 ug. Lane 5: COLO320 Whole Cell Lysate at 40 ug. Lane 6: HELA Whole Cell Lysate at 40 ug. Predicted band size: 27 kD. Observed band size: 27 kD.
EIF6 Antibody - IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-EIF6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
EIF6 Antibody - IHC analysis of EIF6 using anti-EIF6 antibody. EIF6 was detected in immunocytochemical section of SW480 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-EIF6 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
EIF6 Antibody - EIF6 antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.
EIF6 Antibody - EIF6 antibody IHC-paraffin. IHC(P): Mouse Intestine Tissue.
EIF6 Antibody - EIF6 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.
EIF6 Antibody - EIF6 antibody Western blot. All lanes: Anti EIF6 at 0.5 ug/ml. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50 ug. Lane 2: Rat Liver Tissue Lysate at 50 ug. Lane 3: Mouse Liver Tissue Lysate at 50 ug. Lane 4: Human Placenta Tissue Lysate at 50 ug. Lane 5: COLO320 Whole Cell Lysate at 40 ug. Lane 6: HELA Whole Cell Lysate at 40 ug. Predicted band size: 27 kD. Observed band size: 27 kD.
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Polyclonal Rabbit anti‑Human EIF6 Antibody (aa66‑210, IHC, WB) LS‑C407795

Polyclonal Rabbit anti‑Human EIF6 Antibody (aa66‑210, IHC, WB) LS‑C407795

Antibody:
EIF6 Rabbit anti-Human Polyclonal (aa66-210) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407795-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
EIF6 Rabbit anti-Human Polyclonal (aa66-210) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
EIF6 antibody LS-C407795 is an unconjugated rabbit polyclonal antibody to EIF6 (aa66-210) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human EIF6
Synonyms
EIF6 | B(2)GCN homolog | B4 integrin interactor | CAB | EIF-6 | p27(BBP) | p27BBP | B(2)gcn | ITGB4BP
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human EIF6 recombinant protein (Position: N66-T210). Human EIF6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat EIF6.
Epitope
aa66-210
Specificity
Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EIF6
P56537 NM_002212 NP_002203.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 1/15/2025