Mouse EIF4EBP1 / 4EBP1

Rabbit

Mouse, Human, Monkey, Rat, Drosophila (tested or 100% immunogen sequence identity)

IgG Monoclonal

Unconjugated

Ascites

Unmodified

Synthetic phospho-peptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1 (KLH-coupled).

pThr37/Thr46

Recognizes endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. May cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Species cross-reactivity: human, mouse, rat, monkey, and D. melanogaster.

• IHC
• IHC - Paraffin (1:400)
• Immunofluorescence
• Western blot (1:1000)
• Flow Cytometry (1:100)

Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and Flow cytometry. Western Blot: 1:1000. For Western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:400 (Citrate/TBST). Immunofluorescence (IF-IC): 1:50. Flow Cytometry: 1:100.

10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 0.02% Sodium Azide, 50% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q13541 NCBI: NM_004095 NP_004086.1