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Polyclonal Rabbit anti‑Human EIF4E Antibody (phospho‑Ser209, IHC, IF, WB) LS‑C800971
Polyclonal Rabbit anti‑Human EIF4E Antibody (phospho‑Ser209, IHC, IF, WB) LS‑C800971
Antibody:
EIF4E Rabbit anti-Human Polyclonal (pSer209) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Overview
Antibody:
EIF4E Rabbit anti-Human Polyclonal (pSer209) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
EIF4E antibody LS-C800971 is an unconjugated rabbit polyclonal antibody to EIF4E (pSer209) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human EIF4E
Synonyms
EIF4E | EIF-4E | EIF4E1 | EIF4EL1 | EIF4F | CBP | EIF-4F 25 kDa subunit | MRNA cap-binding protein
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen
A synthesized peptide derived from human eIF4E around the phosphorylation site of Serine 209.
Specificity
Phospho-eIF4E (Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at Serine 209.
Applications
- IHC (1:50 - 1:200)
- Immunofluorescence (1:100 - 1:500)
- Western blot (1:500 - 1:2000)
- Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
Featured Products
Species:
Human, Mouse, Rat
Applications:
Western blot, ELISA
Species:
Human
Applications:
IHC, Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
ICC, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 8/31/2024