Target
Human EIF2B5
Synonyms
EIF2B5 | CACH | EIF2BE | EIF-2B | LVWM | CLE | EIF2Bepsilon
Reactivity
Human, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic phosphopeptide derived from the region of rat eIF2B that contains serine 535 (corresponding to serine 539 in the human sequence).
Specificity
Human (80% homologous) and rat eIF2B.
Applications
- Western blot
- Functional Assay
Usage
The antibody has been used in Western blotting. Eukaryotic Initiation Factor 2B (eIF2B) plays a key role in the initiation of mRNA translation in eukaryotic cells by acting as a guanine nucleotide exchange factor. The activity of eIF2B is regulated by a wide array of conditions. Stress inhibits its action, while stimulation of protein synthesis results in its activation. eIF2B, an 82kD subunit of eIF2B, is phosphorylated at serine 539 by glycogen synthase kinase-3 (GSK-3), which leads to inhibition of its guanine nucleotide exchange activity. Positive control: Recombinant eIF2B treated with GSK-3 ; CHO-T cells transfected with human insulin receptor (IR) +/- insulin.
Presentation
1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.2, 0.1% Sodium Azide, 50% Glycerol
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee