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Catalog Number	Size	Price
LS-C353177-100	100 µl (1 mg/ml)	$299

EIF2AK3 / PERK Antibody - Immunohistochemical analysis of PERK staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

EIF2AK3 / PERK Antibody - Immunofluorescent analysis of PERK staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

EIF2AK3 / PERK Antibody - Western blot analysis of PERK expression in HeLa (A) whole cell lysates.

EIF2AK3 / PERK Antibody - Immunoprecipitation of PERK from 0.5mg HeLa whole cell extract lysate using 5ug of Anti-PERK Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-PERK Antibody.

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Polyclonal Rabbit anti‑Human EIF2AK3 / PERK Antibody (N‑Terminus, IHC, IF, WB) LS‑C353177

Name: Anti-EIF2AK3 / PERK Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C353177-100
Price: 299.0000 USD
Availability: InStock

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Antibody:

EIF2AK3 / PERK Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human

Format:

Unconjugated, Unmodified

$299

LS-C353177-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

EIF2AK3 / PERK Rabbit anti-Human Polyclonal (N-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

PERK antibody LS-C353177 is an unconjugated rabbit polyclonal antibody to human PERK (EIF2AK3) (N-Terminus). Validated for ICC, IF, IHC, IP and WB.

Target

Human EIF2AK3 / PERK

Synonyms

See All EIF2AK3 / PERK Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-Terminus of human PERK.

Epitope

N-Terminus

Specificity

Recognizes endogenous levels of PERK protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:10 - 1:100)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About EIF2AK3 / PERK

Uniprot: Q9NZJ5 NCBI: NM_004836 NP_004827.4