Target
Human EIF2AK2 / PKR
Synonyms
EIF2AK2 | p1/eIF-2A protein kinase | p68 kinase | PRKR | EIF-2A protein kinase 2 | p68 | Protein kinase RNA-activated | Tik
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide correspoP9103-74Bnding to residues surrounding Thr451 of human PKR (KLH coupled).
Specificity
Detects endogenous levels of human PKR at 74kD. Does not cross-react with other related proteins.
Applications
- ICC
- Immunofluorescence
- Western blot (1:1000)
- Immunoprecipitation (1:100)
- ELISA
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry (for Immunofluorescence). Western Blot: 1:1000; incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:100. Immunocytochemistry (for IF): 1:50.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee