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Antibodies > EIF2AK2 / PKR > LS-C352730
 
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Catalog Number Size Price
LS-C352730-100 100 µl (1 mg/ml) $299 
EIF2AK2 / PKR Antibody - Immunohistochemical analysis of PKR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EIF2AK2 / PKR Antibody - Immunofluorescent analysis of PKR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
EIF2AK2 / PKR Antibody - Western blot analysis of PKR expression in HeLa (A); MCF7 (B); Caco2 (C) whole cell lysates.
EIF2AK2 / PKR Antibody - Immunohistochemical analysis of PKR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EIF2AK2 / PKR Antibody - Immunofluorescent analysis of PKR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
EIF2AK2 / PKR Antibody - Western blot analysis of PKR expression in HeLa (A); MCF7 (B); Caco2 (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human EIF2AK2 / PKR Antibody (C‑Terminus, IHC, IF, WB) LS‑C352730

Polyclonal Rabbit anti‑Human EIF2AK2 / PKR Antibody (C‑Terminus, IHC, IF, WB) LS‑C352730

Submit Review LSBio Guarantee
Antibody:
EIF2AK2 / PKR Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C352730-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
EIF2AK2 / PKR Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PKR antibody LS-C352730 is an unconjugated rabbit polyclonal antibody to human PKR (EIF2AK2) (C-Terminus). Validated for ICC, IF, IHC and WB.
Target
Human EIF2AK2 / PKR
Synonyms
EIF2AK2 | p1/eIF-2A protein kinase | p68 kinase | PRKR | EIF-2A protein kinase 2 | p68 | Protein kinase RNA-activated | Tik
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human PKR.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of PKR protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EIF2AK2 / PKR
P19525 NM_002759 NP_002750.1

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Featured Products

EIF2AK2 / PKR Antibody - Anti-EIF2AK2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ EIF2AK2 / PKR Antibody LS‑B563
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Product
EIF2AK2 / PKR Antibody (phospho‑Thr451) LS‑C75195
Species: Human, Mouse
Applications: Western blot
EIF2AK2 / PKR Antibody - EIF2AK2 antibody Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
EIF2AK2 / PKR Antibody (aa280‑329) LS‑C117004
Species: Human
Applications: Western blot
Product
EIF2AK2 / PKR Antibody (phospho‑Thr451) LS‑C17287
Species: Human
Applications: IHC, Western blot
Product
EIF2AK2 / PKR Antibody (phospho‑Thr446) LS‑C17480
Species: Human
Applications: Western blot
EIF2AK2 / PKR Antibody - Immunohistochemical analysis of PKR (pT451) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
EIF2AK2 / PKR Antibody (phospho‑Thr451) LS‑C354070
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
See all EIF2AK2 / PKR Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/16/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
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United States

Tel: (800) 227-6666 / (650) 697-3600

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For questions about orders:
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