Human EHMT2 / G9A

Rabbit

Human, Monkey (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein A purified

Unmodified

KLH-conjugated, synthetic peptide (C-DERVDSDSKSEVEALTEQ) corresponding to amino acids 71-88 of human G9a, with an N-Terminus cysteine added for conjugation purposes. The immunizing sequence has 17/18 identical amino acids in mouse. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Mouse, Rat, Elephant, Panda, Bovine, Horse, Rabbit, Pig (94%).

aa71-88

Recognizes human G9a protein, Mr ~140kD. Species cross-reactivity: Human. Predicted to cross-react with mouse based on sequence homology.

• Western blot (0.2 - 2 µg/ml)

Suitable for use in Western Blot. Western Blot: 0.2-2 ug/ml detected G9a in HeLa nuclear extract cell lysate. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-G9a (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q96KQ7 NCBI: NM_006709 NP_006700.3