Target
Human E2F4
Synonyms
E2F4 | E2F-4 | p107/p130-binding protein | Transcription factor E2F4
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
Fusion Protein corresponding to the human E2F-4 protein.
Specificity
Recognizes E2F-4 at 55kD. Species cross-reactivity: Human, mouse and rat.
Applications
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
- ELISA
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 0.5-2 ug/ml detected E2F4 in RIPA lysates from non-stimulated A431 cell lysate. Previous lots have detected E2F4 in HeLa nuclear extract, mouse 3T3 fibroblasts, and rat L6 skeletal myoblasts. Immunoprecipitation: 4-10 ug immunoprecipitated E2F4 from 500 ug of mouse 3T3/A431 cell lysate.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee