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Antibodies > DYKDDDDK Tag > LS-C142551
 
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Catalog Number Size Price
LS-C142551-0.5 0.5 ml $386 
DYKDDDDK Tag Antibody - DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged protein demonstrating that the rat monoclonal L5 antibody is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
DYKDDDDK Tag Antibody - DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged SFO-p41 protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.
DYKDDDDK Tag Antibody - DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged protein demonstrating that the rat monoclonal L5 antibody is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG M2 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
DYKDDDDK Tag Antibody - DYKDDDDK Epitope Tag Antibody (L5) - Western Blot on DYKDDDDK tagged SFO-p41 protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Monoclonal Rat DYKDDDDK Tag Antibody (clone L5, N‑Terminus, IF, WB) LS‑C142551

Monoclonal Rat DYKDDDDK Tag Antibody (clone L5, N‑Terminus, IF, WB) LS‑C142551

Submit Review LSBio Guarantee
Antibody:
DYKDDDDK Tag Rat Monoclonal (N-Terminus) (L5) Antibody
Application:
ICC, IF, WB, IP
Format:
Unconjugated, Unmodified
Other formats:
DY650
Price
Catalog Number
$386
LS-C142551-0.5
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Rat Monoclonal (N-Terminus) (L5) Antibody
Application:
ICC, IF, WB, IP
Format:
Unconjugated, Unmodified
Other formats:
DY650

Specifications

Description
DYKDDDDK Tag antibody LS-C142551 is an unconjugated rat monoclonal antibody to DYKDDDDK Tag (N-Terminus). Validated for ICC, IF, IP and WB.
Target
DYKDDDDK Tag
Host
Rat
Clonality
IgG2a Monoclonal
Clone
L5
Conjugations
Unconjugated. Also available conjugated with DY650.
Purification
Tissue culture supernatant
Modifications
Unmodified
Immunogen
N-Terminus DYKDDDDK-tagged extracellular domain of mouse Langerin
Epitope
N-Terminus
Specificity
DYKDDDDK Epitope Tag (L5).
Applications
  • ICC (1:10)
  • Immunofluorescence (1:10)
  • Western blot (1:1000)
  • Immunoprecipitation (1:1000)
Presentation
PBS, 0.1% Sodium Azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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Customer Reviews (0)

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DYKDDDDK Tag Antibody (clone 29E4.G7, HRP) LS‑C154016
Species:
Applications: IHC, Western blot, ELISA
DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DYKDDDDK Tag Antibody LS‑C413444
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Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
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DYKDDDDK Tag Antibody LS‑C40549
Species:
Applications: Western blot
DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
DYKDDDDK Tag Antibody LS‑C154223
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Species:
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See all DYKDDDDK Tag Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2025
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