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Antibodies > DYKDDDDK Tag > LS-C392574
 
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Catalog Number Size Price
LS-C392574-200 200 µl $393 
DYKDDDDK Tag Antibody - Anti-FLAG Tag Antibody at 1:2000 dilution + 12tag protein lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Monoclonal Mouse DYKDDDDK Tag Antibody (clone 1557CT661.18.1, WB) LS‑C392574

Monoclonal Mouse DYKDDDDK Tag Antibody (clone 1557CT661.18.1, WB) LS‑C392574

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Antibody:
DYKDDDDK Tag Mouse Monoclonal (1557CT661.18.1) Antibody
Application:
WB, Peptide-ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C392574-200
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
DYKDDDDK Tag Mouse Monoclonal (1557CT661.18.1) Antibody
Application:
WB, Peptide-ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
DYKDDDDK Tag antibody LS-C392574 is an unconjugated mouse monoclonal antibody to DYKDDDDK Tag. Validated for Peptide-ELISA and WB.
Target
DYKDDDDK Tag
Host
Mouse
Clonality
IgG1,k Monoclonal
Clone
1557CT661.18.1
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Submit a Publication for LS-C392574

Customer Reviews (0)

Submit a review for LS-C392574

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DYKDDDDK Tag Antibody LS‑C129100
Species:
Applications: Western blot, ELISA
DYKDDDDK Tag Antibody - Western Blot of Mouse antibody for the detection of FLAG™ conjugated proteins Monoclonal Antibody Peroxidase Conjugated. Lane 1: FLAG™ Positive Control Lysates. Load: 10 µg per lane. Primary antibody: FLAG™ Monoclonal Antibody Peroxidase Conjugated at 1:1,000 for overnight at 4°C. Secondary antibody: none.
DYKDDDDK Tag Antibody (clone 29E4.G7, HRP) LS‑C154016
Species:
Applications: IHC, Western blot, ELISA
DYKDDDDK Tag Antibody - Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DYKDDDDK Tag Antibody LS‑C413444
Species:
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation
Product
DYKDDDDK Tag Antibody LS‑C40549
Species:
Applications: Western blot
DYKDDDDK Tag Antibody - Antibody for the detection of FLAG conjugated proteins - Western Blot. Affinity Purified Antibody to detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2500 to detect 1.0 ug of recombinant protein containing either the FLAG epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
DYKDDDDK Tag Antibody LS‑C154223
Species:
Applications: IHC, Western blot, ELISA
Product
DYKDDDDK Tag Antibody (C‑Terminus, AP) LS‑C65173
Species:
Applications: ELISA
See all DYKDDDDK Tag Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2025
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Vector Laboratories, Inc.
6737 Mowry Ave
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United States

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For technical assistance:
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