Target
Rat DRD1 / Dopamine Receptor D1
Synonyms
DRD1 | D(1A) dopamine receptor | DRD1A | DADR | D1a receptor
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
Synthetic peptide 9aa sequence from the rat D1A receptor within the 4th extracellular domain (KLH coupled).
Epitope
4th Extracellular Domain
Specificity
Recognizes the rat dopamine receptor D1a. No significant sequence homology with other dopamine receptors (D1B, D2-D5). Species sequence Homology: human: 88%; porcine: 77%.
Applications
- Western blot (1:500 - 1:2500)
- ELISA (1:5000 - 1:25000)
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 1:500-1:2500 using ECL. Detects 2 proteins of 50 and 75kD in solubilized hippocampal membranes. The 75kD band was not visible in striatum. ~100-400 ug protein/lane should be loaded to visualize the 75kD band. ELISA: 1:5000-1:25000. Coat ELISA plates at 1 ug/ml of Control Peptide.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DRD1 / Dopamine Receptor D1