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Vector Laboratories, Inc.
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Catalog Number Size Price
LS-C60950-2 2 mg (2 mg/ml) $333 
Rabbit IgG Antibody - Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase donkey secondary antibody at 1:1000 for 60 min at RT Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Rabbit IgG. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Donkey Anti‑Rabbit IgG Secondary Antibody (HRP, IHC, WB) LS‑C60950

Polyclonal Donkey Anti‑Rabbit IgG Secondary Antibody (HRP, IHC, WB) LS‑C60950

Antibody:
Rabbit IgG Donkey anti-Rabbit Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA, DB, EM
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$333
LS-C60950-2
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Rabbit IgG Donkey anti-Rabbit Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA, DB, EM
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C60950 is an HRP-conjugated donkey polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB. Cited in 1 publication.
Host
Donkey
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
Applications
  • IHC (1:500 - 1:2000)
  • Western blot (1:1000 - 1:3000)
  • ELISA (1:40000)
  • Dot Blot
  • Electron Microscopy
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

LSBio Ratings

Donkey Anti-Rabbit IgG Secondary Antibody (HRP) for IHC, WB/Western, ELISA LS-C60950 has an LSBio Rating of
Publications (4)

Publications (1)

Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice. Qianyu Zhang, Zhifu Wei, Huinan Weng, Ye Chen, Jie Zhang, Shiwei Mei, Jiahui Wei, Xiulan Zhu, Yingqi Nong, Jianxing Ruan, Wenjuan Liu, Ruiqiong Zhou, Fang Wang, Yanni Xie, Junjiu Huang, Xiqian Zhang, Fenghua Liu. Frontiers in nutrition. 2022 July;9:854655.

Customer Reviews (0)


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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/23/2024