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| Catalog Number | Size | Price |
|---|---|---|
| LS-B800-50 | 50 µg (1 mg/ml) | $460 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human DNMT / DNMT1 Antibody (aa1603‑1616, IHC) LS‑B800
IHC‑plus™ Polyclonal Rabbit anti‑Human DNMT / DNMT1 Antibody (aa1603‑1616, IHC) LS‑B800
Note: This antibody replaces LS-C3010
Antibody:
DNMT / DNMT1 Rabbit anti-Human Polyclonal (aa1603-1616) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
DNMT / DNMT1 Rabbit anti-Human Polyclonal (aa1603-1616) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
DNMT1 antibody LS-B800 is an unconjugated rabbit polyclonal antibody to human DNMT1 (DNMT) (aa1603-1616). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human DNMT / DNMT1
Synonyms
DNMT1 | AIM | CXXC finger protein 9 | CXXC9 | DNA methyltransferase 1 | DNMT | DNA methyltransferase HsaI | DNA MTase HsaI | HSN1E | M.HsaI | MCMT
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human DNMT1.
Epitope
aa1603-1616
Specificity
Amino acids 1603 to 1616 of human DNMT1
Applications
- IHC - Paraffin (10 µg/ml)
- ELISA (1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B800 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B800 was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About DNMT / DNMT1
Validation
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-DNMT1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-DNMT1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
IHC-plus™ DNMT / DNMT1 Antibody (aa1603-1616) for IHC, ELISA LS-B800 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Evidence that active demethylation mechanisms maintain the genome of carcinoma in situ cells hypomethylated in the adult testis. Kristensen DG, Nielsen JE, Jørgensen A, Skakkebæk NE, Rajpert-De Meyts E, Almstrup K. British journal of cancer. 2014 110:668-78. (WB, ELISA; Human)
Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Sheep
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Mouse, Bat, Horse, Rabbit
Applications:
IHC - Paraffin, Western blot, Immunoprecipitation, Chromatin Immunoprecipitation
Species:
Human
Applications:
Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, Immunoprecipitation, ChIP-sequencing
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
