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Catalog Number	Size	Price
LS-C821282-100	100 µg (1 mg/ml)	$528

Dityrosine Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) Dityrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Dityrosine Antibody - Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Dityrosine protein using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.

Dityrosine Antibody - Western Blot analysis of Dityrosine-BSA Conjugate showing detection of 67 kDa Dityrosine protein using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: 3,5-Dibromotyrosine-BSA. Lane 4: Dityrosine-BSA. Lane 5: Bromotyrosine-BSA. Lane 6: 7-ketocholesterol-BSA. Load: 1 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Dityrosine Antibody - Flow Cytometry analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.

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Monoclonal Mouse anti‑All species Dityrosine Antibody (clone 10A6, FITC, IF, WB) LS‑C821282

Name: Anti-Dityrosine Antibody | Mouse anti-All species Monoclonal FITC | LSBio
Brand: Lifespan BioSciences
SKU: LS-C821282-100
Price: 528.0000 USD
Availability: InStock

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Antibody:

Dityrosine Mouse Monoclonal (FITC) (10A6) Antibody

Application:

IF, WB, Flo, ELISA

Reactivity:

All species

Format:

FITC, Unmodified

Other formats:

$528

LS-C821282-100

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

Dityrosine Mouse Monoclonal (FITC) (10A6) Antibody

Application:

IF, WB, Flo, ELISA

Reactivity:

All species

Format:

FITC, Unmodified

Other formats:

Specifications

Description

Dityrosine antibody LS-C821282 is an FITC-conjugated mouse monoclonal antibody to all species Dityrosine. Validated for ELISA, Flow, IF and WB.

Target

All species Dityrosine

See All Dityrosine Antibodies

Host

Mouse

Reactivity

All species

Clonality

IgG1 Monoclonal

Clone

10A6

Conjugations

FITC. Also available conjugated with Atto 390, Atto 488, Atto 594, APC, Biotin, PerCP, RPE.

Purification

Protein G purified

Modifications

Unmodified

Immunogen

Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.

Applications

Immunofluorescence (1:50)
Western blot (1:1000)
Flow Cytometry (1:50)
ELISA (1:1000)
Applications tested for the base form of this product only

Usage

A 1:1000 dilution of the antibody was sufficient for detection of dityrosine in 1 µg of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. Applications should be user optimized.

Presentation

PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol

Storage

Store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee