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Catalog Number Size Price
LS-C358966-30 30 µl (1 mg/ml) $255 
LS-C358966-100 100 µl (1 mg/ml) $299 
LS-C358966-200 200 µl (1 mg/ml) $351 
DDX3 / DDX3X Antibody - Immunohistochemical analysis of DDX3X staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
DDX3 / DDX3X Antibody - Immunofluorescent analysis of DDX3X staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DDX3 / DDX3X Antibody - Western blot analysis of DDX3X expression in HepG2 (A); HeLa (B) whole cell lysates.
DDX3 / DDX3X Antibody - Immunohistochemical analysis of DDX3X staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
DDX3 / DDX3X Antibody - Immunofluorescent analysis of DDX3X staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
DDX3 / DDX3X Antibody - Western blot analysis of DDX3X expression in HepG2 (A); HeLa (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human DDX3 / DDX3X Antibody (Internal, IHC, IF, WB) LS‑C358966

Polyclonal Rabbit anti‑Human DDX3 / DDX3X Antibody (Internal, IHC, IF, WB) LS‑C358966

Antibody:
DDX3 / DDX3X Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C358966-30
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
DDX3 / DDX3X Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DDX3X antibody LS-C358966 is an unconjugated rabbit polyclonal antibody to human DDX3X (DDX3) (Internal). Validated for ICC, IF, IHC and WB.
Target
Human DDX3 / DDX3X
Synonyms
DDX3X | CAP-Rf | DDX14 | DDX3 | DEAD box, X isoform | DBX | Dead box protein 3 | DEAD/H box-3 | Helicase like protein 2 | Helicase-like protein 2 | HLP2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human DDX3X.
Epitope
Internal
Specificity
Recognizes endogenous levels of DDX3X protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DDX3 / DDX3X
O00571 NM_001356 NP_001347.3

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 12/27/2024