Target
Borrelia burgdorferi DbpA
Reactivity
Borrelia burgdorferi
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Lyophilized
Immunogen
MBP-fusion protein corresponding to Borrelia burgdorferi Dbp-A protein.
Specificity
This antibody is specific for Borrelia burgdorferi DbpA protein. A BLAST analysis was used to suggest cross-reactivity with DbpA from Borrelia burgdorferi sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against Borrelia garinii sources based on 60-80% homology. Cross-reactivity with DbpA from other sources has not been determined.
Applications
- Western blot (1:1000)
- ELISA (1:5000)
Usage
This protein-A purified antibody has been tested for use in Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 18.5 kD in size corresponding to Borrelia burgdorferi DbpA protein by Western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee