Target
Human DBH/Dopamine Beta Hydroxylase
Synonyms
DBH | DBM | Dopamine beta-hydroxylase
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
Synthetic peptide, CPTSQGRSPAGPTVVSI-amide, from C-Terminus of human (DbH). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon (94%); Monkey (88%); Horse, Rabbit (82%).
Specificity
Dopamine Beta Hydroxylase (DbH) C-terminal. By immunoblot the antibody specifically reacts with a single (or double) band (s) of MW >70-75000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE. Species Reactivity: By immunoblot: human, monkey, bovine and rabbit (slight).
Applications
- IHC (1:125 - 1:500)
- Western blot (1:125 - 1:500)
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Usage
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:125-1:500 on human, monkey and bovine adrenal medulla. Immunohistochemistry: 1:125-1:500, staining was evaluated in the locus ceruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed strong staining on both human and monkey.
Presentation
10 mM HEPES, pH 7.5, 0.09% sodium azide, 0.1 mg/ml BSA, 50% glycerol, 150mM sodium chloride
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DBH/Dopamine Beta Hydroxylase